IL-6 [Biotinylated] : IL-6 R alpha Inhibitor Screening ELISA Kit

产品详情

ID	Components	Size
EP146-C01	High-bind Plate	1 plate
EP146-C02	Human IL-6 R alpha	15 μg
EP146-C03	Biotinylated Human IL-6	10 μg
EP146-C04	Anti-IL-6 Neutralizing Antibody	10 μg
EP146-C05	Streptavidin-HRP	10 μg
EP146-C06	Coating Buffer	12 mL
EP146-C07	10xWashing Buffer	50 mL
EP146-C08	Blocking Buffer	50 mL
EP146-C09	Substrate Solution	12 mL
EP146-C10	Stop Solution	7 mL

产品概述（Product Overview）
IL-6 [Biotinylated] : IL-6 R alpha Inhibitor Screening ELISA Kit is based on a competition-ELISA method, is used to screening for inhibitors of human IL-6binding to human IL-6 R. The kit is designed to provide a reliable solution for the screening of antibodies and small molecule inhibitors in the early stages, as well as QC release, to help researchers quickly screen and validate drugs, effectively improving research and development efficiency. It can also be used as a universal detection tool to identify the ability of human IL-6binding to human IL-6 R.
重构方法（Reconstitution）
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
存储（Storage）
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
质量管理控制体系（QMS）

典型数据-Typical Data
Please refer to DS document for the assay protocol.
INHIBITION OF IL-6 [Biotinylated]: IL-6 R alpha BINDING BY ANTI-IL6 NEUTRALIZING ANTIBODY
Serial dilutions of Anti-IL-6 Neutralizing antibody (Catalog # EP146-C04) (1:1 serial dilution, from 10 μg/mL to 0.01μg/mL) was added into Biotinylated IL-6: IL-6 R alpha binding reactions. The assay was performed according to the protocol described below. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).

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