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Human latent GDF-8 Protein, His Tag (MALS verified)

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GD8-H5343-50ug
¥3675.00

GD8-H5343-500ug
¥25200.00
合计0件 产品金额¥ 0

产品详情

  • 分子别名(Synonym)

    GDF-8, Myostatin, GDF8, MSTN, Growth, differentiation factor 8

  • 表达区间及表达系统(Source)

    Human latent GDF-8 Protein, His Tag (GD8-H5343) is expressed from CHO cells. It contains AA Asn 24 - Ser 375 (Accession # O14793-1).

    Predicted N-terminus: Asn 24

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 42.0 kDa. The protein migrates as 10-11 kDa and 38-40 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

    The protein is designed as a dimer.

  • 内毒素(Endotoxin)

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • 纯度(Purity)

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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数据展示

  • 电泳(SDS-PAGE)

    GDF-8 SDS-PAGE

    Human latent GDF-8 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    GDF-8 SEC-MALS

    The purity of Human latent GDF-8 Protein, His Tag (Cat. No. GD8-H5343) is more than 90% and the molecular weight of this protein is around 78-108 kDa verified by SEC-MALS.

    Report
  • 活性(Bioactivity)-ELISA

     GDF-8 ELISA

    Immobilized Human latent GDF-8 Protein, His Tag (Cat. No. GD8-H5343) at 5 μg/mL (100 μL/well) can bind Human Activin RIIB Protein, Fc Tag (Cat. No. ACB-H5254) with a linear range of 0.06-8 ng/mL (QC tested).

    Protocol
  •  GDF-8 ELISA

    Immobilized Human latent GDF-8 Protein, His Tag (Cat. No. GD8-H5343) at 5 μg/mL (100 μL/well) can bind Biotinylated Human Activin RIIB Protein, Fc,Avitag (Cat. No. ACB-H82F3) with a linear range of 0.1-16 ng/mL (Routinely tested).

    Protocol
  •  GDF-8 ELISA

    Immobilized Human latent GDF-8 Protein, His Tag (Cat. No. GD8-H5343) at 5 μg/mL (100 μL/well) can bind Human Follistatin Protein, Fc Tag (Cat. No. FON-H5256) with a linear range of 0.2-31 ng/mL (Routinely tested).

    Protocol

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背景介绍

Growth differentiation factor 8 (GDF8), also known as myostatin, is a unique member of the transforming growth factor-β superfamily that is expressed in human granulosa cells and has important roles in regulating a variety of ovarian functions. GDF8 acts as a negative regulator of skeletal muscle growth and differentiation. In addition to the expression in the musculoskeletal system, GDF8 is also expressed in various tissues, including the reproductive system.

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