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Biotinylated Human LY6G6D Protein, His,Avitag™ (HPLC verified)

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  • 分子别名(Synonym)

    lymphocyte antigen 6 family member G6D,C6orf23,G6D,LY6-D,MEGT1,NG25

  • 表达区间及表达系统(Source)

    Biotinylated Human LY6G6D Protein, His,Avitag (LYD-H82Q3) is expressed from human 293 cells (HEK293). It contains AA Asn 20 - Ser 104 (Accession # O95868-1).

    Predicted N-terminus: N/A

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    LY6G6D Structure

    This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 12.8 kDa. The protein migrates as 15-18 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 偶联(Conjugate)

    Biotin

  • 标记(Labeling)

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • 内毒素(Endotoxin)

    Less than 1.0 EU per μg by the LAL method.

  • 纯度(Purity)

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-HPLC.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

数据展示

  • 电泳(SDS-PAGE)

    LY6G6D SDS-PAGE

    Biotinylated Human LY6G6D Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    LY6G6D SEC-HPLC

    The purity of Biotinylated Human LY6G6D Protein, His,Avitag (Cat. No. LYD-H82Q3) was greater than 90% as determined by SEC-HPLC.

  • 活性(Bioactivity)-ELISA

     LY6G6D ELISA

    Immobilized Biotinylated Human LY6G6D Protein, His,Avitag (Cat. No. LYD-H82Q3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Mouse Anti-LY6G6D Antibody (3D6E10), IgG1 with a linear range of 0.004-0.125 μg/mL (QC tested).

    Protocol

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背景介绍

LY6G6D belongs to a cluster of leukocyte antigen-6 (LY6) genes located in the major histocompatibility complex (MHC) class III region on chromosome 6. Members of the LY6 superfamily typically contain 70 to 80 amino acids, including 8 to 10 cysteines. Most LY6 proteins are attached to the cell surface by a glycosylphosphatidylinositol (GPI) anchor that is directly involved in signal transduction.

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