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PE-Labeled Mouse SIRP alpha Protein, His Tag (Site-specific conjugation)

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  • 分子别名（Synonym）

    SHPS1,SIRPA,CD172A,BIT,MFR,MYD1,P84,PTPNS1

  • 表达区间及表达系统（Source）

    PE-Labeled Mouse SIRP alpha, His Tag (SIA-MP2H6) is produced via site-specific conjugation of PE to Mouse SIRP alpha, His Tag under optimal conditions with a proprietary technology. Mouse SIRP alpha, His Tag is expressed from human 293 cells (HEK293). It contains AA Lys 32 - Asn 373 (Accession # P97797-1).

    Predicted N-terminus: Lys 32

    Request for sequence

  • 蛋白结构（Molecular Characterization）

    SIRP alpha Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 41.5 kDa.

  • 偶联（Conjugate）

    PE

    Excitation Wavelength: 488 nm / 561 nm

    Emission Wavelength: 575 nm

  • 应用说明（Application）

    Please note that this product is NOT compatible to streptavidin detection system.

  • 制剂（Formulation）

    Lyophilized from 0.22 μm filtered solution in PBS, 0.5% BSA, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法（Reconstitution）

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储（Storage）

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.

  • 质量管理控制体系（QMS）

    1. 质量管理体系（ISO, GMP）
    2. 质量优势
    3. 质控流程

数据展示

  • 活性（Bioactivity）-FACS

    SIRP alpha FACS

    5e5 of overexpressing mouse CD47 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of PE-Labeled Mouse SIRP alpha, His Tag (Cat. No. SIA-MP2H6) and negative control protein respectively. PE signal was used to evaluate the binding activity (QC tested).

    Protocol

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背景介绍

Tyrosine-protein phosphatase non-receptor type substrate 1 (SHPS1) is also known as CD172 antigen-like family member A (CD172a), Macrophage fusion receptor, MyD-1 antigen, Signal-regulatory protein alpha (SIRPA or SIRP alpha) or p84, is a member of the SIRP family, and also belongs to the immunoglobulin superfamily. SIRP alpha is Ubiquitous and highly expressed in brain. SIRPA / CD172a is immunoglobulin-like cell surface receptor for CD47 and acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPA / SHPS-1 supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment and may play a key role in intracellular signaling during synaptogenesis and in synaptic function By similarity. SIRPA / MyD1 involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin and mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.

前沿进展

 
药物研发进展
  • 英文全称：

    Signal-regulatory protein alpha

  • 中文全称：

    信号调节蛋白α

  • 种类：

  • 上市药物数量：

    2 详情

  • 临床药物数量：

    18 详情

  • 最高研发阶段：

    批准上市

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