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分子别名（Synonym）
VCAM1,CD106,INCAM-100,V-CAM 1,VCAM-1
表达区间及表达系统（Source）
Biotinylated Human VCAM-1, Fc,Avitag, premium grade (VC1-H82F6) is expressed from human 293 cells (HEK293). It contains AA Phe 25 - Glu 698 (Accession # P19320-1).
Predicted N-terminus: Phe 25
It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 102.4 kDa. The protein migrates as 130 kDa±5 kDa under reducing (R) condition, and ＞250 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
偶联（Conjugate）
Biotin
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
内毒素（Endotoxin）
Less than 0.01 EU per μg by the LAL method.
蛋白A残留（Protein A）
5 ppm of protein tested by ELISA.
宿主蛋白残留（Host Cell Protein）
0.5 ng/µg of protein tested by ELISA.
宿主核酸残留（Host Cell DNA）
0.02 ng/μg of protein tested by qPCR.
无菌（Sterility）
Negative
支原体（Mycoplasma）
Negative.
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 24 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
数据展示
电泳（SDS-PAGE）
Biotinylated Human VCAM-1, Fc,Avitag, premium grade on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Human VCAM-1, Fc,Avitag, premium grade (Cat. No. VC1-H82F6) is more than 90% and the molecular weight of this protein is around 210-250 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human ITGA4&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W1) at 5 μg/mL (100 μL/well) can bind Biotinylated Human VCAM-1, Fc,Avitag, premium grade (Cat. No. VC1-H82F6) with a linear range of 0.1-13 ng/mL (QC tested).
Protocol
Immobilized Biotinylated Human VCAM-1, Fc,Avitag, premium grade (Cat. No. VC1-H82F6) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human ITGA4&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W1) with a linear range of 0.02-0.313 μg/mL (Routinely tested).
Protocol
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