resDetect™ Biotinylated Human DLL4 ELISA Kit (Residue Testing)

产品参数（Product Specifications）

产品概述（Product Overview）

resDetect™ Biotinylated Human DLL4 ELISA Kit (Residue Testing) is based on the ELISA indirect method and is used to detect and quantitatively determine GMP biotinylated human DLL4 residues in cell culture supernatants, serum, and plasma. The kit contains GMP biotinylated human DLL4 (ACROBiosystems, cat# GMP-DL4H23) to ensure accurate assay results and is designed to provide a reliable solution for CAR-T product quality assessment during drug development and CMC quality control stages. It can also be used as a universal detection tool for the quantitative determination of biotinylated human DLL4.

应用说明（Application）

0

It is for research use only.

重构方法（Reconstitution）

Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.

存储（Storage）

2-8℃

组分（Materials Provided）

原理（Assay Principles）

This assay kit employs a standard indirect-ELISA format, providing a rapid detection of Biotinylated Human DLL4. The kit consists of Pre-coated Anti-DLL4 Antibody Microplate and Biotinylated Human DLL4 Standard and Streptavidin-HRP and buffers.

Your experiment will include 5 simple steps:

a) Bring all reagents to room temperature(20℃-25℃) before use.

b) Add your sample to the plate and take the Biotinylated Human DLL4 Standard. The samples and standard are diluted by Dilution Buffer.

c) Wash the plate and add the Streptavidin-HRP diluted by Dilution Buffer to the plate.

d) Wash the plate and add TMB.

e) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated by the absorbance at 450 nm minus the absorbance at 630 nm to remove background disturbance before statistical analysis. The OD Value reflects the amount of bound protein.

质量管理控制体系（QMS）

1. 质量管理体系（ISO, GMP）
2. 质量优势
3. 质控流程