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PD-1 [Biotinylated] : PD-L1 Inhibitor Screening ELISA Kit

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货号-规格
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EP-101-96tests
¥6300.00
合计0件 产品金额¥ 0

产品详情

  • 组分(Materials Provided)

    ID
    Components
    Size
    EP101-C01
    High-bind Plate
    1 plate
    EP101-C02
    Human PD-L1
    25 μg
    EP101-C03
    Anti-PD-1 Neutralizing Antibody
    20 μg
    EP101-C04
    Human PD-1-Biotin
    5 μg
    EP101-C05
    Streptavidin-HRP
    10 μg
    EP101-C06
    Coating Buffer
    12 mL
    EP101-C07
    10xWashing Buffer
    50 mL
    EP101-C08
    Blocking Buffer
    50 mL
    EP101-C09
    Substrate Solution
    12 mL
    EP101-C10
    Stop Solution
    7 mL

  • 产品概述(Product Overview)

    PD-1 [Biotinylated] : PD-L1 Inhibitor Screening ELISA Kit is based on a competition-ELISA method, is used to screening for inhibitors of human PD-1 binding to human PD-L1. The kit is designed to provide a reliable solution for the screening of antibodies and small molecule inhibitors in the early stages, as well as QC release, to help researchers quickly screen and validate drugs, effectively improving research and development efficiency. It can also be used as a universal detection tool to identify the ability of human PD-1 binding to human PD-L1.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.

  • 存储(Storage)

    1. Unopened kit should be stored at 2℃-8℃ upon receiving.

    2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

    3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

数据展示

  • 典型数据-Typical Data

    Please refer to DS document for the assay protocol.

     PD-1 & PD-L1 TYPICAL DATA

    INHIBITION OF PD-1-PD-L1 BINDING BY ANTI-PD-1 NEUTRALIZING ANTIBODY (CATALOG # EP101-C03) MEASURED USING THE PD-1 [BIOTINYLATED] : PD-L1 INHIBITOR SCREENING ELISA ASSAY PAIR (CATALOG # EP-101).
    Anti-PD-1 neutralizing antibody was diluted from 10 μg/mL to 0.078 μg/ mL (69.628 nM to 0.544 nM) and loaded onto the plate coated by human PD-L1 in the presence of human PD-1-Biotin. Assay was performed according to the protocol in PD-1[Biotinylated] : PD-L1 Inhibitor Screening ELISA Assay Pair. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).

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