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Human CD27 Ligand / CD70 Protein, Fc Tag, active trimer (MALS verified)

COADSMS
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CDL-H5266-100ug
¥3865.00
CDL-H5266-1mg (500ug X 2)
¥25475.00
合计0件 产品金额¥ 0
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产品详情

  • 分子别名（Synonym）

    CD70,CD27LG,TNFSF7,TNFSF7G,CD27L

  • 表达区间及表达系统（Source）

    Human CD27 Ligand Protein, Fc Tag (CDL-H5266) is expressed from human 293 cells (HEK293). It contains AA Ser 52 - Pro 193 (Accession # P32970-1) trimer Design.

    Predicted N-terminus: Pro

  • 蛋白结构（Molecular Characterization）

    This protein carries a human IgG1 Fc tag at the N-terminus.

    The protein has a calculated MW of 74.1 kDa. The protein migrates as 130-150 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

  • 偶联（Conjugate）

    Unconjugated

  • 内毒素（Endotoxin）

    Less than 1.0 EU per μg by the LAL method.

  • 纯度（Purity）

    >95% as determined by SDS-PAGE.

  • 制剂（Formulation）

    Lyophilized from 0.22 μm filtered solution in Tris, Glycine and NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法（Reconstitution）

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储（Storage）

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.

  • 质量管理控制体系（QMS）

    1. 质量管理体系（ISO, GMP）
    2. 质量优势
    3. 质控流程

数据展示

  • 电泳（SDS-PAGE）

    CD27 Ligand SDS-PAGE

    Human CD27 Ligand Protein, Fc Tag on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • SEC-MALS

    CD27 Ligand SEC-MALS

    The purity of Human CD27 Ligand Protein, Fc Tag (Cat. No. CDL-H5266) is more than 85% and the molecular weight of this protein is around 160-190 kDa verified by SEC-MALS.

    Report

  • 活性（Bioactivity）-ELISA

    CD27 Ligand ELISA

    Immobilized Human CD27, His Tag (Cat. No. CD7-H522b) at 2 μg/mL (100 μL/well) can bind Human CD27 Ligand Protein, Fc Tag (Cat. No. CDL-H5266) with a linear range of 0.2-25 ng/mL (QC tested).

    Protocol

  • CD27 Ligand ELISA

    Serial dilutions of Anti-CD27 Ligand (cusatuzumab) Antibody, Human IgG1 were added into Human CD27 Ligand Protein, Fc Tag (Cat. No. CDL-H5266): Biotinylated Human CD27, Fc,Avitag (Cat. No. TN7-H82F6) binding reactions. The half maximal inhibitory concentration (IC50) is 0.74034 μg/mL (Routinely tested).

    Protocol

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背景介绍

Cluster of Differentiation 70 (CD70) is also known as CD27 ligand (CD27L / CD27LG), TNFSF7, TNFSF7G, is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF) and is a surface antigen found on activated T-and B-lymphocytes and mature dendritic cells. Binding of CD70 to its receptor CD27 induces in priming, effector functions, differentiation and memory formation of T-cells, and thus is invloved in the biological processes including T-cell activation, the proliferation of costimulated T-cells, as well as the generation of cytolytic T-cells. CD70 on T cells provides costimulatory signals that are required for T cell proliferation, clonal expansion and the promotion of effector T cell formation. CD70 on mouse B cell has been shown to inhibit terminal differentiation of activated B cells into plasma cells and enhances commitment to memory B cell responses. CD70 induces proliferation and IFNγ production, on NK cells.

前沿进展

 
药物研发进展
  • 英文全称：

    CD70 antigen

  • 中文全称：

    CD70抗原

  • 种类：

  • 上市药物数量：

    0 详情

  • 临床药物数量：

    30 详情

  • 最高研发阶段：

    临床二期

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