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分子别名（Synonym）
MAdCAM-1,MAdCAM1,Mucosal vascular addressin cell adhesion molecule 1 transcript variant 1
表达区间及表达系统（Source）
Human MAdCAM-1, His Tag (MAM-H52H4) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Gln 317 (Accession # Q13477-1).
Predicted N-terminus: Gln 19
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 33.3 kDa. The protein migrates as 46-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联（Conjugate）
Unconjugated
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
90% as determined by SDS-PAGE.
95% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
产品展示
电泳（SDS-PAGE）
Human MAdCAM-1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Human MAdCAM-1, His Tag (Cat. No. MAM-H52H4) is more than 95% and the molecular weight of this protein is around 45-60 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-SPR
Human MAdCAM-1, His Tag (Cat. No. MAM-H52H4) immobilized on CM5 Chip can bind Human ITGA4&ITGB7 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT7-H52W4) with an affinity constant of 0.227 μM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
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